permeabilization solution (Servicebio Inc)

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Servicebio Inc permeabilization solution
Permeabilization Solution, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/permeabilization+solution/permeabilization/pmc13030181-114-24-26
Average 86 stars, based on 1 article reviews

permeabilization solution - by Bioz Stars, 2026-10

86/100 stars

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Transfection:

Article Title: LincRNA-P21 knockdown facilitates esophageal squamous cell carcinoma cell progression by upregulating cadherin 5 via YTH domain containing 1
Article Snippet: Protein bands were measured using Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific). .. Cells were transfected with si-NC, si-lincRNA-P21, or si-lincRNA-P21+si-CDH5 for 48 h. Fixed cells on slides were permeabilized with permeabilization solution (Servicebio) for 20 min at room temperature. .. Cells were then blocked with 3% BSA for 30 min at room temperature and incubated overnight with primary antibodies against PCNA (1:500, GB11010; Servicebio), collagen I (1:800, GB11022; Servicebio) or fibronectin (1:200, GB114057; Servicebio) at 4°C.

Article Title: LincRNA-P21 knockdown facilitates esophageal squamous cell carcinoma cell progression by upregulating cadherin 5 via YTH domain containing 1.
Article Snippet: Protein bands were measured using Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific). .. Immunofluorescence assay Cells were transfected with si-NC, si-lincRNA-P21, or si-lincRNAP21+si-CDH5 for 48 h. Fixed cells on slides were permeabilized with permeabilization solution (Servicebio) for 20 min at room temperature. .. Cells were then blocked with 3% BSA for 30 min at room temperature and incubated overnight with primary antibodies against PCNA (1:500, GB11010; Servicebio), collagen I (1:800, GB11022; Servicebio) or fibronectin (1:200, GB114057; Servicebio) at 4°C.

Article Title: Long noncoding RNAs with peptide‐encoding potential identified in esophageal squamous cell carcinoma: KDM4A‐AS1 ‐encoded peptide weakens cancer cell viability and migratory capacity
Article Snippet: .. At 48 h after transfection, cells climbing slides were permeabilized with permeabilization solution (Servicebio) and blocked with 3% BSA. .. Next, cells were incubated with anti‐FASN primary antibody (cat. no. 10624‐2‐AP, Proteintech, Wuhan, China) for almost 12 h at 4 °C and FITC‐conjugated goat‐anti‐mouse secondary antibody (cat.no.

Immunofluorescence:

Incubation:

Article Title: Yuyang Decoction Regulates Macrophage Polarization and Repairs the Intestinal Mucosal Barrier via the TLRs/Tollip Signaling Pathway
Article Snippet: .. The cells were treated with permeabilization solution (G1204, 0.5%, Servicebio) and incubated at room temperature for 10 minutes. .. After serum blocking, primary antibodies against claudin-1 (1:1000, 13,050-1-ap, Proteintech) and ZO-1 (1:1000, 21,773-1-ap, Proteintech) were applied, and the cells were incubated at 4°C overnight.

other:

Article Title: Tree Shrew Genome-Wide CRISPR Screen Identifies RNF6 as a Proviral Host Factor for Zika Virus Replication in Brain Microvascular Endothelial Cells
Article Snippet: Primary antibodies were visualized using HRP-conjugated anti-mouse IgG (ZenBio, Chengdu, China, 511103, 1:15,000) or anti-rabbit IgG (ZenBio, Chengdu, China, 511203, 1:15,000).

Permeability:

Article Title: Programmable engineered bacteria as sustained-releasing antibody factory in situ for enhancing tumor immune checkpoint therapy.
Article Snippet: The outer membrane vesicles (OMVs) of four groups were collected via a bacterial MV isolation kit (Umibio, China). .. Permeabilization solution (Servicebio, China) was added for 15 min to improve the permeability. ..

Review